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To determine the role of GpoA during pneumococcal infection, we constructed the gpoA mutant in the capsulated D39 strain (serotype 2), which is virulent in BALB/c mice using intranasal inoculation [38], and its corresponding revertant strain ( wt gpoA + )

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Part of the spinal cord gray and white matter was used for analysis under light microscopy (magnification 300)