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Similarly, incretin-like actions of GIP may also proceed indirectly through amino acids and potentiation of GIPR-dependent stimulation of glucagon secretion from alpha cells [47]

Occasional exposure to room temperature is not always harmful

The resulting cell lysate was filtered through a 0.2-m filter and loaded onto a 5-mL HisTrap HP column (GE Healthcare/Cytiva) equilibrated in wash buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM TCEP, 25 mM imidazole)