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Another study in larger cohorts has been conducted to profile metabolic alterations and disordered metabolic pathways for hyperuricemia and gout by LCMS analysis, and further discovered 13 metabolites to distinguish hyperuricemia and gout from normouricemic controls [18]

The resulting samples were crosslinked with a limiting amount of glutaraldehyde at 0.075% for 25 min at room temperature and quenched with 100 mM Tris-HCl pH 8.0 for 5 min

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